Page 227 - 北京京煤集团总医院第十届·2022学术年会论文集

北京京煤集团总医院 第十届·2022 学术年会论文集

Figure 1. Flowchart of this study.

2. Materials and methods

2.1. Data collection
A total of 110 samples were included in the GSE147507 dataset, among which 78 samples,

comprising 55 uninfected with SARS-CoV-2 and 23 SARS-CoV-2 infected samples, were analyzed

in this study. In addition, transcriptomic profiling of the GSE143303 dataset, including 47

endobronchial biopsy samples of patients with severe asthma and 13 healthy controls, was

downloaded from the GEO database [11].

2.2. Data preprocessing and differential expression analysis

The RStudio software (version 4.1.2) [12] was used to normalize the transcriptome data. The

DEGs were screened from GSE147507 and GSE143303 datasets using limma [13] and DESEq2

[14] packages with a cutoff value of P < 0.01 and |logFC| > 0, respectively. The overlapping DEGs

screened from the GSE147507 and GSE143303 datasets were then extracted using Jvenn [15].

2.3. Enrichment analysis

Based on the overlapping DEGs, the Enrichr database [16] was used to conduct GO and KEGG

- 222 -