Page 225 - 北京京煤集团总医院第十届·2022学术年会论文集

北京京煤集团总医院 第十届·2022 学术年会论文集

Exploration of potential biomarkers and mechanisms for COVID-19 and asthma based on

microarray analysis

Yaning Gao1, Jian Li1*, Liang Chen1, Zhengjun Wen1
1 Beijing Jingmei Group General Hospital, Beijing, China

* Correspondence: jhhhcb@163.com;
†These authors contributed equally.

Abstract: The aim of this study is to investigate the potential mechanisms of coronavirus disease

(COVID-19) and asthma comorbidities. GSE147507 and GSE143303 datasets were obtained from

the GEO database, the differential expressed genes (DEGs) were identified, and the overlapping

DEGs were obtained by determining the DEG intersection between the two datasets. A series of

analyses of the shared DEGs were performed, including enrichment analysis, PPI network

construction, construction of TF/miRNA-gene interaction networks, and drug-gene and disease-

gene interactions. A total of 135 overlapping DEGs were obtained by determining the DEGs

intersection between the GSE147507 and GSE143303 datasets. These overlapped DEGs were

significantly enriched in the regulation of DNA-templated transcription, initiation, clathrin-sculpted

gamma-aminobutyric acid transport vesicle, DNA binding, and eight KEGG pathways. The PPI
network revealed that HSPA8, SRSF1, NDUFAB1, PTEN, CCT8, HIST1H2BK, HIST2H2BE, DLAT,

EIF3G, and WAC, with high scores, were the hub genes. In addition, 65 TFs and 369 miRNAs
targeted overlapping DEGs. Finally, these overlapped DEGs were also related to other diseases,

such as hyperglycemia, metabolic acidosis, and lung neoplasm, and the top 10 drugs with the most

significant potential included lanatoside C, digoxin, GW-8510, doxorubicin, daunorubicin,

proscillaridin, anisomycin, helveticoside, ouabain, and bisacodyl. HSPA8, SRSF1, NDUFAB1,

PTEN, CCT8, HIST1H2BK, HIST2H2BE, DLAT, EIF3G, WAC, FOXC1, GATA2, hsa-miR-93-5p,

and hsa-miR-17-5p may play vital roles in COVID-19/asthma comorbidity. Lanatoside C, digoxin,

GW-8510, doxorubicin, daunorubicin, proscillaridin, anisomycin, helveticoside, ouabain, and

bisacodyl may serve as drug targets against COVID-19/asthma comorbidity.

Keywords: COVID-19, asthma, protein–protein interaction network, microRNA, bioinformatics

analysis

1. Introduction

Since the outbreak of coronavirus disease-2019 (COVID-19) towards the end of 2019, COVID-

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