Page 261 - 北京京煤集团总医院第十一届·2023学术年会论文集

北京京煤集团总医院 第十一届·2023 学术年会论文集

荧光免疫法和 2 种化学发光法检测血清总 β-HCG 结果的

比对分析

崔雯，姜慧英，王鹏飞，李丽艳（北京京煤集团总医院检验科）
【摘要】目的：通过比较同一实验室 3 种不同方法学检测系统荧光免疫法（星童 Pylon 3D）、

电化学发光法（罗氏 e601）和酶促化学发光法（迈瑞 CL-6000i）测定血清总 β-HCG 的结
果，探讨 3 种方法检测 β-HCG 的是否具有可比性，为不同仪器测定实验结果互认提供依

据。方法：选取不同浓度常规血清标本 186 份，先在罗氏 e601 电化学发光系统进行检测，

再用星童 Pylon 3D 荧光免疫法和迈瑞 CL-6000i 酶促化学发光法检测系统进行检测。以罗氏

e601 电化学发光检测系统作为参考仪器进行数据比对。结果：3 种发方法学之间检测结果的

相关性都在 0.99 以上，相关性良好。随着 β-HCG 浓度范围的增高，3 种发方法学检测结果

的相关性，都呈现出越来越高的趋势。总体一致性分析，迈瑞的数值与罗氏较为靠近，偏倚

均数 Bias=-528.3 mIU/mL，是最低的，另外两组的偏倚均数都在负 1000 mIU/mL 以上。结

论：3 种检测系统对总 β-HCG 检测结果之间具有良好的相关性和一致性。

【关键词】人绒毛膜促性腺激素，不同方法学，相关性，一致性

The Comparison and analysis of serum total β-HCG detected by fluorescence immunoassay

and two chemiluminescence methods
[Abstract] Objective: To compare the results of serum total β-HCG measured by fluorescence

immunoassay (Pylon 3D), electrochemiluminescence assay (Roche E601) and enzymatic
chemiluminescence assay (Mindray CL-6000I) with three different methods in the same laboratory,

and to explore whether the three methods are comparable in the detection of β-HCG. It provides a

basis for mutual recognition of experimental results measured by different instruments. Methods:

A total of 186 routine serum samples with different concentrations were detected by Roche E601

electrochemiluminescence system, and then by Pylon 3D fluorescent immunoassay and Mindray

CL-6000I enzymatic chemiluminescence detection system. Roche E601 electrochemiluminescence

detection system was used as the reference instrument for data comparison. Results: The correlation

between the three methods was above 0.99, and the correlation was good. With the increase of β-

HCG concentration range, the correlation of the three methods showed an increasing trend. The

overall consistency analysis showed that Mindray's value was close to Roche's, with Bias mean =-

528.3 mIU/mL, which was the lowest, while the Bias mean of the other two groups were all above

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